Tuesday, August 16, 2011

What would happen if DNA Pol I lost its 5' to 3' exonuclease activity?

This will help me answer one of my homework questions. So I know that Pol I is the only of the bacterial polymerases with the 5' to 3' exonuclease activity that allows it to remove ribonucleotide primers and replace with deoxyribonucleotides. So I gather then that as cell division continues, the chromosome of a bacterium would contain RNA...because the primers for the lagging strand and origin couldn't be excised. Would this affect replication itself? Or would it still proceed....and would it maybe affect gene expression somehow? Thanks!

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